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goat anti bmp14  (Novus Biologicals)


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    Structured Review

    Novus Biologicals goat anti bmp14
    Goat Anti Bmp14, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 91/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/goat+anti+bmp14/Mouse+GDF-5%2FBMP-14+Antibody/10__3390_slash_livers3020019-72-25-29
    Average 91 stars, based on 9 article reviews
    goat anti bmp14 - by Bioz Stars, 2026-11
    91/100 stars

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    Protein Extraction:

    Article Title: Expression of Bone Morphogenetic Protein 14 in Liver Disease and Cancer
    Article Snippet: Quantitative real-time PCR was performed by applying the LightCycler 480 System (Roche Diagnostics, Mannheim, Germany) as described [17] and the following sets of specific primers: α-SMA human (forward: 5′-CGT GGC TAT TCC TTC GTT AC-3′, reverse: 5′-TGC CAG CAG ACT CCA TCC-3′), β-actin human (forward: 5′-CTA CGT CGC CCT GGA CTT CGA GC-3′, reverse: 5′-GAT GGA GCC GCC GAT CCA CAC GG-3′), β-actin murine (forward: 5′-AGG CCA ACC GTG AAA AGA T-3′, reverse: 5′-GGC GTG AGG GAG AGC ATA-3′), BMP14 human (forward: 5′-TGT ACA GGA CGC TGT CCG A-3′, reverse: 5′-ACA CGT ACC TCT GCT TCC TGA-3′), BMP14 murine (forward: 5′-TTG ACA AAG GGC AAG ATG ACC GA-3′, reverse: 5′-AAC ACC TCC CAG CCA GAT CC-3′), COL1A1 human (forward: 5′-CGG CTC CTG CTC CTC TT-3′, reverse: 5′-GGG GCA GTT CTT GGT CTC-3′), ID1 human (forward: 5′-GTA TCT GCT TCG GGC TTC CA-3′, reverse: 5′-TGA TTC TTG GCG ACT GGC T-3′) and ID2 human (forward: 5′-CAC GGA TAT CAG CAT CCT GTC CT-3′, reverse 5′-CAA GTA AGA GAA CAC CCT GGG AAG A-3′). .. Protein extraction from cells and Western blotting were performed as described [18] by applying the following primary antibodies: rabbit anti-αSMA (ab32575, 1:1000, Abcam, Cambridge, UK), goat anti-BMP14 (AF853, 1:2000, Novus Biologicals, Wiesbaden, Germany), rabbit anti-GAPDH (#2118, 1:1000, Cell Signaling Technology, Danvers, MA, USA) and rabbit anti-ID1 (sc-1331-04, 1:100, Santa Cruz Biotechnology, Heidelberg, Germany). .. Donkey anti-goat (sc-2304, 1:5000, Santa Cruz Biotechnology, Heidelberg, Germany) and mouse anti-rabbit (sc-2357, 1:10,000, Santa Cruz Biotechnology, Heidelberg, Germany) were used as secondary antibodies.

    Western Blot:

    Article Title: Expression of Bone Morphogenetic Protein 14 in Liver Disease and Cancer
    Article Snippet: Quantitative real-time PCR was performed by applying the LightCycler 480 System (Roche Diagnostics, Mannheim, Germany) as described [17] and the following sets of specific primers: α-SMA human (forward: 5′-CGT GGC TAT TCC TTC GTT AC-3′, reverse: 5′-TGC CAG CAG ACT CCA TCC-3′), β-actin human (forward: 5′-CTA CGT CGC CCT GGA CTT CGA GC-3′, reverse: 5′-GAT GGA GCC GCC GAT CCA CAC GG-3′), β-actin murine (forward: 5′-AGG CCA ACC GTG AAA AGA T-3′, reverse: 5′-GGC GTG AGG GAG AGC ATA-3′), BMP14 human (forward: 5′-TGT ACA GGA CGC TGT CCG A-3′, reverse: 5′-ACA CGT ACC TCT GCT TCC TGA-3′), BMP14 murine (forward: 5′-TTG ACA AAG GGC AAG ATG ACC GA-3′, reverse: 5′-AAC ACC TCC CAG CCA GAT CC-3′), COL1A1 human (forward: 5′-CGG CTC CTG CTC CTC TT-3′, reverse: 5′-GGG GCA GTT CTT GGT CTC-3′), ID1 human (forward: 5′-GTA TCT GCT TCG GGC TTC CA-3′, reverse: 5′-TGA TTC TTG GCG ACT GGC T-3′) and ID2 human (forward: 5′-CAC GGA TAT CAG CAT CCT GTC CT-3′, reverse 5′-CAA GTA AGA GAA CAC CCT GGG AAG A-3′). .. Protein extraction from cells and Western blotting were performed as described [18] by applying the following primary antibodies: rabbit anti-αSMA (ab32575, 1:1000, Abcam, Cambridge, UK), goat anti-BMP14 (AF853, 1:2000, Novus Biologicals, Wiesbaden, Germany), rabbit anti-GAPDH (#2118, 1:1000, Cell Signaling Technology, Danvers, MA, USA) and rabbit anti-ID1 (sc-1331-04, 1:100, Santa Cruz Biotechnology, Heidelberg, Germany). .. Donkey anti-goat (sc-2304, 1:5000, Santa Cruz Biotechnology, Heidelberg, Germany) and mouse anti-rabbit (sc-2357, 1:10,000, Santa Cruz Biotechnology, Heidelberg, Germany) were used as secondary antibodies.



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    91
    Novus Biologicals goat anti bmp14
    Goat Anti Bmp14, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/goat+anti+bmp14/Mouse+GDF-5%2FBMP-14+Antibody/10__3390_slash_livers3020019-72-25-29
    Average 91 stars, based on 1 article reviews
    goat anti bmp14 - by Bioz Stars, 2026-11
    91/100 stars
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